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Clontech pabai

WebDec 2, 2024 · The Y1H Gold strain is the compatible strain for pABAi vector provided by Clontech. Can we use Ym4271 (the strain for Y1H assay and has the same genotype of Y1H Gold strain) instead of Y1H Gold ... WebApr 11, 2024 · The yeast one-hybrid assay was performed using a Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, USA). ... (Gao et al. 2015) was amplified from NIP genomic DNA and inserted into pAbAi vector to generate bait vectors. After that, the bait vectors were linearized and introduced into the Y1H Gold yeast to create reporter ...

Identification of JAZ-interacting MYC transcription factors involved …

WebMar 1, 2024 · The plasmids [pAbAi-pPks1, pAbAi-pEthD and pAbAi-pMlac1] were linearized and cloned onto Y1H Gold cells (Clontech, USA). Transformed strains were grown on SD-Ura agar medium. Subsequently, the MrAbaA coding region was amplified from cDNA and cloned onto the linearized pGADT7 vector (Clontech, USA). The … WebApr 16, 2024 · Background This study aimed to identify the transcription factors of nitrate reductase genes (NIA1 and NIA2) promoters and hypothetical cis-element of NRE2. Based on the constructed cDNA library of Nicotiana tabacum K326, a yeast one-hybrid system was established using the Matchmaker® Gold Yeast One-Hybrid Library Screening System … screws for fastening wood to metal https://usl-consulting.com

Clontech Laboratories, Inc. is now Takara Bio USA, Inc.

http://www.biofeng.com/zaiti/jiaomu/pAbAi.html WebAug 14, 2015 · zt624 pAbAi Clontech 酵母单杂交系统. zt625 pBiFC-bFosVC155 Addgene BiFC 蛋白相互作用检测系统. zt626 pBiFC-bFos(deltaZIP)VC155 Addgene BiFC 蛋白相互作用检测系统. zt627 mCerulean-N1 Addgene FRET 蛋白相互作用检测系统. zt628 mCerulean -C1 Addgene FRET 蛋白相互作用检测系统 WebNov 29, 2024 · The positive recombinant clones were identified by PCR using Matchmaker Insert Check PCR Mix 1 (Clontech, CA, USA). The empty pAbAi vector was identified … screws for floating shelves

A retrotransposon insertion in the Mao1 promoter results in erect ...

Category:BoMyrosinase plays an essential role in sulforaphane accumulation …

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Clontech pabai

Construction of yeast one-hybrid library and screening of …

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Clontech pabai

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WebThe Y1H Gold strain is the compatible strain for pABAi vector provided by Clontech. Can we use Ym4271 (the strain for Y1H assay and has the same genotype of Y1H Gold … WebY1HGold菌株是Clontech公司开发的GAL4-AbA酵母单杂系统用菌株，MATα型，可直接转化质粒进行筛库试验。Transformation m arker为：ura3，leu2；报告基因为：AbAr。Y1HGold-GAL4-AbA 酵母单杂系统需要两种质粒配套使用：pAbAi 和PGADT7。

WebFeb 28, 2024 · the pAbAi vector (Clontech). pAbAi constructs were then. separately integrated into the genome of the Y1HGold strain. by homologous recombination to generate six bait reporter. strains. Web2 days ago · The amplified product was inserted into the pAbAi vector (Clontech, Palo Alto, CA, USA) to generate a proBoMY-pAbAi construct. The coding sequence of BoFAMA was cloned into the pGADT7 vector to generate pGADT7::BoFAMA recombinant construct. The primers are listed in Table S1. Subsequently, the constructs were transformed into yeast …

WebCLONTECH Laboratories, Inc.Tel:800 662 2566 Fax:800 424 1350 Email:[email protected] Address:1290 Terra Bella Avenue Mountain View, CA … Webthe pAbAi vector (Clontech). pAbAi constructs were then separately integrated into the genome of the Y1HGold strain by homologous recombination to generate six bait reporter strains. WRKY2-and ...

WebJul 25, 2024 · The pAbAi-ProAtFT and pGADT7-AaZFP3 vectors were cotransformed into the yeast Y1HGold strain using a Matchmaker One-Hybrid Library Construction and Screening Kit (Clontech). Cotransformed yeast cells were selected on SD/-Ura-Leu (a synthetic dropout medium lacking Ura and Leu) with or without AbA (0.6 mg L −1 ; Sigma …

WebMar 3, 2024 · The RT300 sequence of QH34 was amplified and cloned into the pAbAi vector to form the pBait-AbAi plasmid, Y1H-RT300. The Y1H-RT300 plasmid was integrated into the genome of yeast ... (Clontech). For high stringency in the interaction tests to the RT300 sequence, ... screws for fixing wood to metalWebMar 19, 2024 · Three fragments (PF1, PF2, and PF3) enriched with two adjacent W-boxes and three corresponding mutant fragments (MPF1, MPF2, and MPF3) with mutant W-boxes of the promotor of MYB97 were independently cloned into the pAbAi vector (Clontech). pAbAi constructs were then separately integrated into the genome of the Y1HGold strain … pay my fpl bill western unionWebNov 27, 2024 · Cloning of transcription factors (TFs) We employed the Yeast One-Hybrid assay (Y1H) (Clontech Y1H, Clontech, USA) to identify TFs that interact with the cloned promoters. Briefly, promoter sequences were cloned into pAbAi Vector (Clontech Y1H, Clontech, USA) to construct the bait vectors. To provide the “prey”, floral-derived cDNA … pay my foxtel bill onlineWebMar 1, 2024 · Matchmaker Gold 单杂交文库筛选过程直接、快速、简单！. 第一步：将1-3个目标DNA序列（bait）重复串联后克隆到pAbAi中。. 第二步：将线性化的pBait-AbAi载 … pay my franklin county taxesWebJul 1, 2016 · When sending email, please continue to use "clontech.com" as our domain name. We will transition to "takarabio.com" by the end of 2016. Thank you for your time … screws for foam insulationWebY1H assay was performed using Matchmatch TM Gold Yeast One-Hybrid System (Clontech) according to the manufacturer’s instructions. LOG4, CKX7, GA2ox4, and GA2ox5 promoters were ligated into the pAbAi vector and the full-length coding region of BRC1 was also amplified using specific primers ( SI Appendix , Table S8 ) and fused to … screws for glasses united supermarketWebUSD $185.00. Matchmaker Insert Check PCR Mix 1 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, dNTPs and buffer; simply add cells to the mix … pay my fpl with a credit card