Phis-sumo vector

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Webbyeast 2-hybrid vectors (sundquist lab) pGADT7 and pGBKT7. Ligation Independent Cloning (LIC) vectors from Mark Connelly: pMCSG10, pMCSG7, pMCSG17-20, pMCSG19, … Webbvariable. A vector xi 2Rm can be assigned one of four disjoint point sets y1, y2, y3 or y4 within an m-dimensional feature space. xi 2Rm is the ith training data row and yi …

Champion™ pET SUMO Expression System

Webb8 aug. 2024 · For the GST pull-down assay, full-length GI was cloned into the pHis-SUMO vector, and the sequence encoding the cytoplasmic tail of SOS1 (residues 453 to 1,146) … Webb28 dec. 2024 · To determine which specific SUMO form is responsible for IQGAP1 SUMOylation, three kinds of SUMO tags (pHis-SUMO1, pHis-SUMO2 and pHis-SUMO3) ... grand max soft plus

Y187-pHis2YeastOne-HybridMediaKit 使用说明书

WebbChampion Pet Sumo Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … WebbDownload PHIS database for standalone from PHISDetector webserver and check MD5 Due to the large size of the database file (~100G), we recommend using -c (continue) … WebbSmt3, hereby referred to as SUMO, is a member of a ubiquitin-like protein family that regulates several cellular processes including apoptosis, nuclear transport, and cell cycle … grandmax interior

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WebbFounded in 1996, LifeSensors, Inc. helps life scientists innovate in basic research, drug discovery and diagnostics. LifeSensors pioneered the SUMO-fusion system for high … WebbChampion™ pET SUMO 表达系统作为完整的系统提供。Champion™ pET SUMO TA Cloning™ 盒含有线性化 Champion™ pET SUMO 载体、无菌水、dNTP、10X PCR 缓冲 … grandmax surveyors limitedWebbpGADT7 AD is a yeast two-hybrid expression vector that is designed to express a fusion protein between the GAL4 activation domain (AD; amino acids 768–881) and a protein of interest. The fusion protein is expressed at high levels from the full-length ADH1 promoter. The fusion protein also contains a hemagglutinin (HA) epitope tag. chinese foyer table

"Webb28 sep. 2024 · Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV … " - Phis-sumo vector

Phis-sumo vector

Sumo Vector Images (over 1,200) - VectorStock

WebbIncludes: The Champion pET SUMO TA Cloning box contains linearized Champion pET SUMO vector, sterile water, dNTPs, 10X PCR Buffer, control template and primers, T4 DNA ligase, 10X ligation buffer, primers for sequencing or PCR screening, and an expression control. The SUMO Protease box contains SUMO Protease and buffers. Webbsmall size of the pRham N-His SUMO Kan Vector (2.6 kb) facilitates cloning of large inserts and performing DNA manipulations, such as site- directed mutagenesis. The pRham N …

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WebbCentrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. … Webbencoding amino acids 1-96 of SUMO-1 (lacking glycine 97 creating a SUMO-1 that is not susceptible to proteolytic removal by isopeptidases) was fused in frame to the amino …

WebbWelcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free … WebbOur SUMO-tag expression systems maximize the yield of soluble, functional proteins in E. coli. SUMO functions as both a chaperonin and initiator of protein folding, improving the …

WebbThe best selection of Royalty Free Sumo Vector Art, Graphics and Stock Illustrations. Download 1,200+ Royalty Free Sumo Vector Images. Webb26 maj 2024 · 载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically …

Webb7 juli 2014 · Hexahistidine small ubiquitin-like modifier (SUMO) tagged constructs were generated by cloning the ANKS3-SAM and ANKS6-SAM sequences into a pHis-SUMO …

WebbThe pSUMO vector is a low copy plasmid with kanamycin selection. ( b ) The DNA sequence encoding the SUMO tag. The restriction digestion site of enzyme BsaI is … chinesefreecodeWebb第1 页共2 页 Y187-pHis2YeastOne-HybridMediaKit使用说明书产品编号：YM1010（序号1-8），YM1011（YM1010+SL0930）储存温度：序号1-8常温保存；序号9需-20℃储存， … grand max phonehttp://www.coolaber.com/usr/2160/File/F/F4671.pdf chinese fox dramasWebb25 okt. 2012 · SUMO (smallubiquitinlikemodifier 是一种小分子泛素样修饰蛋白 ,广泛存在于各种真核细胞中,参与调节细胞凋亡、信号转导、RNA 转录、蛋白的核质运输以及细胞周 … grandmax tweakers bluetoothWebbExamples of UBLs are SUMO, Rub1 (also called Nedd8), ISG15, Apg8 and Apg12. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA … chinese framinghamWebbVector type Bacterial Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Low Copy … chinese freecodecamp.orgWebbThe IsdH expression vector used in this study was a modi- ﬁed version of the pRM208 plasmid, which contained the second and third NEAT domains and the intervening … grandmax station